Journal: bioRxiv

Article Title: Temporal Tracking of Plasma Cells in vivo Using J-chain CreERT2 Reporter System

doi: 10.1101/2023.12.02.569736

Figure Lengend Snippet: A. Schematic of the IgJCre ERT2 locus. B. Experimental setup for the in vitro differentiation of plasma cells using LPS and 4-Hydroxy-tamoxifen (4-OHT). B cells from IgJCre ERT2 , IgJCre ERT2 tdTomato+, and littermate controls were isolated by CD43 depletion, stimulated with LPS, and evaluated by flow cytometry. Representative flow plots show: C. GFP fluorescence versus cell trace violet proliferation dye; D. tdTomato fluorescence versus cell trace violet proliferation dye. E. PCs were defined by CD138 expression and were further analyzed for F. GFP and tdTomato expression.

Article Snippet: Single-cell suspensions of spleens were prepared and subjected to CD43 depletion (Invitrogen: 11422D).

Techniques: In Vitro, Isolation, Flow Cytometry, Fluorescence, Expressing

Journal: bioRxiv

Article Title: Temporal Tracking of Plasma Cells in vivo Using J-chain CreERT2 Reporter System

doi: 10.1101/2023.12.02.569736

Figure Lengend Snippet: A. B cells from IgJCre ERT2 , IgJCre ERT2 tdTomato + , and littermate controls were isolated via CD43 depletion and stimulated with LPS, or BAFF. Following flow cytometry analysis, cells were evaluated for GFP fluorescence versus Cell Trace Violet proliferation dye, tdTomato fluorescence versus Cell Trace Violet proliferation dye, and plasma cells were identified by CD138 expression and further analyzed for GFP and Tomato expression. B. Data from multiple in vitro experiments in which CD43 depleted B cells from IgJCre ERT2 , IgJCre ERT2 tdTomato + , and littermate controls were stimulated with LPS. Each data point is the average of 2-3 technical replicates.

Article Snippet: Single-cell suspensions of spleens were prepared and subjected to CD43 depletion (Invitrogen: 11422D).

Techniques: Isolation, Flow Cytometry, Fluorescence, Expressing, In Vitro